Study of the Square Archaeon Haloquadratum walsbyi Using Atomic Force Microscopy
Author Information
Author(s): Sublimi Saponetti Matilde, Bobba Fabrizio, Salerno Grazia, Scarfato Alessandro, Corcelli Angela, Cucolo Annamaria
Primary Institution: University of Salerno
Hypothesis
What are the morphological features of the archaeon Haloquadratum walsbyi as observed through atomic force microscopy?
Conclusion
The study revealed new morphological details of Haloquadratum walsbyi, including the presence of a surface layer protein lattice and an external capsule.
Supporting Evidence
- The study demonstrated the presence of a regular corrugation with a periodicity of 16–20 nm attributed to the S-layer protein lattice.
- Images showed the evolution of the cell during the drying process, indicating the presence of an external capsule.
- AFM analysis revealed the presence of gas vesicles and poly-β-hydroxybutyrate granules within the cells.
Takeaway
Scientists looked at a tiny square microorganism called Haloquadratum walsbyi using a special microscope to see its surface and found some interesting features like a protective layer.
Methodology
The study used amplitude-modulation atomic-force microscopy to analyze the cell surface of Haloquadratum walsbyi in nearly physiological conditions.
Limitations
The study faced challenges in imaging cells in liquid due to low adhesion and potential cell agglomeration.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website