Effects of seven days’ fasting on physical performance and metabolic adaptation during exercise in humans
2025

Effects of Seven Days of Fasting on Physical Performance and Metabolic Adaptation

Sample size: 13 publication 10 minutes Evidence: moderate

Author Information

Author(s): Kolnes Kristoffer J., Nilsen Emelie T. F., Brufladt Steffen, Meadows Allison M., Jeppesen Per B., Skattebo Øyvind, Johansen Egil I., Birk Jesper B., Højlund Kurt, Hingst Janne, Skålhegg Bjørn S., Kjøbsted Rasmus, Griffin Julian L., Kolnes Anders J., O’Rahilly Stephen, Wojtaszewski Jørgen F. P., Jensen Jørgen

Primary Institution: Norwegian School of Sport Sciences, Oslo, Norway

Hypothesis

How does seven days of fasting affect physical performance and metabolic adaptation during exercise in humans?

Conclusion

Maximal leg muscle strength was preserved during fasting, but maximal oxygen uptake and work capacity decreased by about 15%.

Supporting Evidence

  • Participants lost an average of 4.6 kg of lean mass and 1.4 kg of fat mass after fasting.
  • Maximal isometric and isokinetic strength remained unchanged after fasting.
  • Peak oxygen uptake decreased by 13% after fasting.
  • Muscle glycogen was halved during the fasting period.
  • PDK4 expression increased 13-fold during fasting.

Takeaway

When people fast for a week, they can still be strong, but they might get tired more easily when exercising.

Methodology

Thirteen participants completed a seven-day water-only fasting intervention, with daily evaluations and physical performance tests before and after fasting.

Potential Biases

Participants were all healthy young adults, which may not represent the general population.

Limitations

The study lacked a randomized control group and had a relatively small sample size, limiting generalizability.

Participant Demographics

Thirteen participants (7 males and 6 females), aged approximately 29.7 years.

Statistical Information

P-Value

p<0.00001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1038/s41467-024-55418-0

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