Gene expression changes in Spodoptera frugiperda due to polydnavirus infection
Author Information
Author(s): M. Barat-Houari, F. Hilliou, F-X. Jousset, L. Sofer, E. Deleury, J. Rocher, M. Ravallec, L. Galibert, P. Delobel, R. Feyereisen, P. Fournier, A-N. Volkoff
Primary Institution: UMR 1231 Biologie Intégrative et Virologie des Insectes, INRA – Université de Montpellier II
Hypothesis
Does the injection of the polydnavirus HdIV induce changes in the gene transcript levels of the lepidopteran host Spodoptera frugiperda?
Conclusion
The presence of HdIV induces variations in transcript levels of specific host genes, which may alter the physiology of the parasitized host.
Supporting Evidence
- 24% of the 1750 arrayed host genes showed changes in their transcript levels after HdIV injection.
- 76% of the affected genes displayed decreased transcript levels.
- Quantitative RT-PCR confirmed the microarray results for several genes involved in immunity.
Takeaway
When a virus is injected into caterpillars, it changes how their genes work, which can make it harder for them to fight off the virus.
Methodology
A cDNA microarray was used to analyze gene transcription profiles in hemocytes and fat body tissues of Spodoptera frugiperda after injection of the polydnavirus HdIV.
Limitations
The study may not account for all potential confounding factors affecting gene expression.
Participant Demographics
Last instar larvae of Spodoptera frugiperda were used in the study.
Statistical Information
P-Value
0.0056
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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