Effects of Probiotics on Gut Health in Diabetic Rats
Author Information
Author(s): Nelios Grigorios, Prapa Ioanna, Mitropoulou Gregoria, Kompoura Vasiliki, Balafas Evangelos, Kostomitsopoulos Nikolaos, Yanni Amalia E., Kourkoutas Yiannis
Primary Institution: Democritus University of Thrace
Hypothesis
The study aims to examine the effect of free or immobilized Lacticaseibacillus rhamnosus OLXAL-1 cells on oat flakes on the gut microbiota and metabolic markers in a Type-1 Diabetic animal model.
Conclusion
The study found that while probiotic supplementation did not significantly affect body weight or plasma glucose levels, it did increase beneficial gut bacteria and reduce harmful bacteria in diabetic rats.
Supporting Evidence
- Probiotic administration increased the relative abundances of Lactobacillus and Bifidobacterium.
- Oat flakes supplementation significantly reduced harmful microbial populations.
- IL-1β levels in diabetic animals were lowered with oat flakes supplementation.
- Probiotic strategies may help manage dysbiosis and inflammation in Type 1 diabetes.
Takeaway
This study looked at how adding certain good bacteria to the diets of diabetic rats can help their gut health, even if it doesn't change their weight or blood sugar much.
Methodology
The study involved 48 male Wistar rats divided into eight groups, with different diets including control, oat-supplemented, and diets with free or immobilized L. rhamnosus OLXAL-1 cells, over a 4-week period.
Limitations
The use of an STZ-induced model may not fully reflect naturally occurring Type 1 diabetes in humans, and the sample size for NGS analysis was limited.
Participant Demographics
Male Wistar rats, aged fifteen weeks.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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