Gene Identification in Water Buffalo Using TGG Repeats
Author Information
Author(s): Sudeep Kumar, Ruchi Gupta, Sudhir Kumar, Sher Ali
Primary Institution: National Institute of Immunology, New Delhi, India
Hypothesis
Can TGG repeats in the water buffalo genome help identify genes related to fertility and other traits?
Conclusion
The study identified several genes in the water buffalo that show tissue-specific expression, particularly in the testes and spermatozoa.
Supporting Evidence
- 38 distinct mRNA transcripts were identified from somatic tissues and gonads.
- 15 mRNA transcripts were identified from spermatozoa.
- High expression levels of certain transcripts were found in testes and spermatozoa.
Takeaway
Researchers looked at special DNA sequences in water buffalo to find important genes that might help with breeding better animals.
Methodology
Minisatellite associated sequence amplification (MASA) was used to analyze cDNA from various tissues and spermatozoa.
Limitations
Not all identified genes were characterized, and the study focused on only two genes of clinical significance.
Participant Demographics
Tissue samples were collected from both sexes of water buffalo.
Digital Object Identifier (DOI)
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