Exploring glycopeptide-resistance in Staphylococcus aureus: a combined proteomics and transcriptomics approach for the identification of resistance-related markers
2006

Understanding Glycopeptide Resistance in Staphylococcus aureus

publication Evidence: moderate

Author Information

Author(s): Scherl Alexander, François Patrice, Charbonnier Yvan, Deshusses Jacques M, Koessler Thibaud, Huyghe Antoine, Bento Manuela, Stahl-Zeng Jianru, Fischer Adrien, Masselot Alexandre, Vaezzadeh Alireza, Gallé Francesca, Renzoni Adriana, Vaudaux Pierre, Lew Daniel, Zimmermann-Ivol Catherine G, Binz Pierre-Alain, Sanchez Jean-Charles, Hochstrasser Denis F, Schrenzel Jacques

Primary Institution: Biomedical Proteomics Research Group, Geneva University, Geneva, Switzerland

Hypothesis

What molecular targets are involved in glycopeptide resistance in Staphylococcus aureus?

Conclusion

The study identifies numerous proteins and genes involved in glycopeptide resistance, providing potential targets for diagnostics and treatment.

Supporting Evidence

  • The study identified 94 differentially expressed genes and 178 proteins related to glycopeptide resistance.
  • Massively parallel methods allowed the identification of one third of the complete proteome.
  • The findings suggest that glycopeptide resistance is a stable phenomenon acquired by Staphylococcus aureus.

Takeaway

Scientists studied bacteria that resist antibiotics to find out how they do it, which could help in treating infections better.

Methodology

The study used whole-genome microarray-based transcription and quantitative proteomic profiling on isogenic strains of Staphylococcus aureus.

Limitations

The difficulty in obtaining isogenic strains with various susceptibility phenotypes limits the study's findings.

Digital Object Identifier (DOI)

10.1186/1471-2164-7-296

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