Artificially Induced Epithelial-Mesenchymal Transition in Surgical Subjects
Author Information
Author(s): Aoyagi Kazuhiko, Minashi Keiko, Igaki Hiroyasu, Tachimori Yuji, Nishimura Takao, Hokamura Norikazu, Ashida Akio, Daiko Hiroyuki, Ochiai Atsushi, Muto Manabu, Ohtsu Atsushi, Yoshida Teruhiko, Sasaki Hiroki
Primary Institution: National Cancer Center Research Institute
Hypothesis
Is the gene expression profile of surgical samples useful for predicting clinical outcomes compared to biopsy samples?
Conclusion
The study suggests that artificially induced epithelial-mesenchymal transition in surgical samples may lead to misinterpretation of prognostic evaluations in cancer research.
Supporting Evidence
- Artificially induced epithelial-mesenchymal transition was found in surgical samples.
- 90.9% of surgical samples clustered together in gene expression analysis.
- A significant number of genes were differentially expressed between biopsy and surgical samples.
Takeaway
When doctors take samples from cancer patients, the way they do it can change the results, making it hard to tell how bad the cancer is.
Methodology
Gene expression profiles were analyzed from 166 samples (77 biopsy and 89 surgical) using microarrays.
Potential Biases
Potential contamination of normal tissue in surgical samples may skew results.
Limitations
The study may not account for all variables affecting gene expression in surgical samples.
Participant Demographics
Patients with esophageal cancer, both malignant and normal tissues were analyzed.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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