Comparing DNA Sources for Canine Genetic Studies
Author Information
Author(s): Gonzalo Rincon, Katarina Tengvall, Janelle M Belanger, Laetitia Lagoutte, Juan F Medrano, Catherine André, Anne Thomas, Cynthia Taylor Lawley, Mark ST Hansen, Kerstin Lindblad-Toh, Anita M Oberbauer
Primary Institution: University of California, Davis
Hypothesis
Is buccal-derived DNA as effective as blood-derived DNA for genome-wide association studies in dogs?
Conclusion
Both buccal and blood-derived DNA samples are suitable for genome-wide association studies.
Supporting Evidence
- 97% of samples had SNP call rates over 80%.
- Concordance between buccal and blood samples averaged over 99%.
- Buccal-derived DNA was shown to be a reliable source for genetic studies.
Takeaway
You can collect DNA from a dog's mouth instead of just from their blood, and it works just as well for studying their genes.
Methodology
The study compared DNA from buccal cytobrushes and blood samples using the Illumina Infinium CanineHD Genotyping BeadChip.
Limitations
The study may not account for all potential sources of contamination in buccal samples.
Participant Demographics
Samples were collected from various breeds, including Bearded collies and Standard poodles, with both male and female dogs included.
Statistical Information
Statistical Significance
p > 0.2
Digital Object Identifier (DOI)
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