Stat3 and c-Myc Binding in Embryonic Stem Cells
Author Information
Author(s): Benjamin L. Kidder, Jim Yang, Stephen Palmer
Primary Institution: EMD Serono Research Institute, Inc.
Hypothesis
To further understand ES cell transcriptional networks, we used genome-wide chromatin immunoprecipitation and microarray analysis (ChIP-chip) to map Stat3 and c-Myc binding targets in ES cells.
Conclusion
Stat3 and c-Myc occupy a significant number of genes whose expression is highly enriched in embryonic stem cells.
Supporting Evidence
- Stat3 and c-Myc bind many ES cell enriched genes.
- Stat3 binds both active and inactive genes in ES cells.
- c-Myc binds predominantly active genes in ES cells.
- Stat3 targets are differentially expressed in ES cells following removal of LIF.
Takeaway
This study shows that two important proteins, Stat3 and c-Myc, help keep stem cells in a state where they can become any type of cell.
Methodology
Genome-wide chromatin immunoprecipitation and microarray analysis (ChIP-chip) were used to map Stat3 and c-Myc binding targets in embryonic stem cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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