How Piezo1 Affects Neuronal Growth and Movement in the Gut
Author Information
Author(s): Moneme Chioma, Olutoye Oluyinka O. II, Sobstel Michał F., Zhang Yuwen, Zhou Xinyu, Kaminer Jacob L., Hsu Britney A., Shen Chengli, Mandal Arabinda, Li Hui, Yu Ling, Balaji Swathi, Keswani Sundeep G., Cheng Lily S.
Primary Institution: University of Virginia
Hypothesis
Piezo1 plays an important role in the growth and development of the enteric nervous system (ENS).
Conclusion
The Piezo1 mechanoreceptor may play an important role in the ENS as its activation leads to decreased neuronal growth and migration.
Supporting Evidence
- Stretching neurons led to shorter neurite lengths compared to control.
- Piezo1 activation decreased neuronal migration and recovery from injury.
- Piezo1 inhibition increased neuronal migration.
Takeaway
This study found that a protein called Piezo1 can stop nerve cells in the gut from growing and moving properly, which might be important for understanding gut diseases.
Methodology
Enteric neural crest-derived progenitor cells (ENPC) were isolated from adult mouse intestine and propagated in culture as neurospheres, then subjected to stretch and treated with Piezo1 agonist or antagonist.
Potential Biases
Potential bias due to the use of specific animal models and the limitations of in vitro studies.
Limitations
The study's use of Piezo1 antagonists may have off-target effects, and the RNA sequencing may lack precision in heterogeneous cell populations.
Participant Demographics
Male and female C57BL/6J mice aged 3–8 weeks.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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