GAP-43 Interactions with Other Proteins in Neurons
Author Information
Author(s): Ollom Callise M., Denny John B.
Primary Institution: University of Texas Health Science Center at San Antonio
Hypothesis
GAP-43 binds to various proteins in growing neurons as part of its mechanism of action.
Conclusion
GAP-43 does not extensively interact with cytoskeletal proteins in differentiated N1E-115 cells, but it may interact with calmodulin.
Supporting Evidence
- GAP-43 was not found in the 100,000 × g pellet fractions, indicating it does not sediment with actin.
- Calmodulin co-immunoprecipitated with GAP-43 following crosslinker treatment.
- The majority of GAP-43 in growing N1E-115 cells is not complexed to other proteins.
Takeaway
GAP-43 is a protein that helps neurons grow, but in this study, it didn't stick to the usual partners like actin; instead, it mostly hung out with calmodulin.
Methodology
Differentiated N1E-115 neuroblastoma cells were treated with a crosslinking reagent and analyzed by immunoprecipitation and two-dimensional gel electrophoresis.
Limitations
The study does not confirm the presence of GAP-43 in complexes with actin or other proteins, leaving open the possibility of untested interactions.
Digital Object Identifier (DOI)
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