Measuring Endosomal pH in Dictyostelium
Author Information
Author(s): Anna Marchetti, Emmanuelle Lelong, Pierre Cosson
Primary Institution: University of Geneva, Faculty of Medicine
Hypothesis
Can we accurately measure the endosomal pH in Dictyostelium using flow cytometry and specific fluorescent probes?
Conclusion
The study presents a method to accurately assess the endosomal pH of Dictyostelium lysosomes, revealing their extreme acidity.
Supporting Evidence
- The study utilized a pH-sensitive probe, Oregon green, to measure endosomal pH.
- Results indicated that the pH of early endocytic compartments (lysosomes) was very acidic, below 3.5.
- Flow cytometry allowed for single-cell measurements of endosomal pH.
- The method described is simpler and requires fewer cells compared to traditional spectrofluorimeter methods.
Takeaway
This study shows how scientists can use special dyes to find out how acidic the insides of certain cells are, which helps us understand how these cells work.
Methodology
The study used flow cytometry with Oregon green and pH-insensitive markers to measure endosomal pH in individual Dictyostelium cells.
Limitations
The method is not ideal for precise determination of post-lysosomal pH.
Digital Object Identifier (DOI)
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