Genetic Diversity in TB Isolates from Ghana
Author Information
Author(s): Yeboah-Manu Dorothy, Asante-Poku Adwoa, Bodmer Thomas, Stucki David, Koram Kwadwo, Bonsu Frank, Pluschke Gerd, Gagneux Sebastien
Primary Institution: Noguchi Memorial Institute for Medical Research, University of Ghana
Hypothesis
The study aims to investigate the population structure of Mycobacterium tuberculosis complex isolates in South-Western Ghana using spoligotyping and large sequence polymorphism.
Conclusion
The study found that 36.4% of TB cases in South-Western Ghana are caused by the Cameroon sub-lineage of MTBC and 20% by M. africanum type 1.
Supporting Evidence
- 80.25% of isolates were identified as Mycobacterium tuberculosis sensu stricto.
- 19.75% of isolates were classified as Mycobacterium africanum type 1.
- MTBss isolates were more likely to be resistant to streptomycin and any drug resistance compared to M. africanum.
Takeaway
This study looked at different types of germs that cause tuberculosis in Ghana and found that some are more common than others.
Methodology
The study used spoligotyping and large sequence polymorphism (LSP) to analyze MTBC isolates from sputum samples collected from TB patients.
Limitations
The study's sample size may not fully represent the diversity of TB strains in all regions of Ghana.
Participant Demographics
Participants included 109 males (67.7%) and 52 females (32.3%), with ages ranging from 2 to 90 years.
Statistical Information
P-Value
p<0.03
Confidence Interval
95%CI=1.0004–7.35
Statistical Significance
p<0.008
Digital Object Identifier (DOI)
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