Reference Intervals for Biochemical and Hematological Parameters in Mice
Author Information
Author(s): Mazzaccara Cristina, Labruna Giuseppe, Cito Gennaro, Scarfò Marzia, De Felice Mario, Pastore Lucio, Sacchetti Lucia
Primary Institution: Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II and CEINGE-Biotecnologie Avanzate S.C.aR.L.
Hypothesis
What are the reference values for biochemical and hematological parameters in commonly used mouse strains across different ages and genders?
Conclusion
The study provides age- and gender-specific reference intervals for biochemical and hematological parameters in C57BL/6J, 129SV/EV, and C3H/HeJ mice.
Supporting Evidence
- The study found significant inter-strain differences in biochemical parameters.
- Gender differences were observed in hematological parameters across all strains.
- Age-dependent variations were recorded for most biochemical parameters.
- Reference intervals were calculated using non-parametric methods.
- Quality control procedures were implemented to ensure data accuracy.
Takeaway
This study helps scientists know what normal blood test results look like for different types of mice at different ages and genders.
Methodology
The study measured 26 biochemical analytes and 5 hematological parameters in 90 mice (30 per strain) across three age ranges using dry chemistry and automated blood counters.
Potential Biases
Potential bias due to the specific strains and controlled conditions under which the mice were kept.
Limitations
The study may not account for all possible variations in mouse strains and environmental factors affecting the results.
Participant Demographics
30 mice per strain, with equal numbers of male and female mice across three age ranges: 1-2 months, 3-8 months, and 9-12 months.
Statistical Information
P-Value
p range: 0.05–0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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