Host ABCE1 is at Plasma Membrane HIV Assembly Sites and Its Dissociation from Gag is Linked to Subsequent Events of Virus Production
2007
ABCE1 and HIV Assembly
publication
Evidence: high
Author Information
Author(s): Julia E. Dooher, Bobbie L. Schneider, Jonathan C. Reed, Jaisri R. Lingappa
Primary Institution: University of Washington
Hypothesis
Whether Gag–ABCE1 complexes constitute true assembly intermediates remains controversial.
Conclusion
ABCE1 associates with Gag during HIV assembly and dissociates before virus maturation.
Supporting Evidence
- ABCE1 is recruited to sites of assembling Gag at the plasma membrane.
- Dissociation of ABCE1 from Gag correlates with Gag processing during virion maturation.
- Gag–ABCE1 complexes increase in size to that of immature capsids.
Takeaway
ABCE1 is a helper protein that works with HIV to build new virus particles, and it leaves just before the virus is ready to be released.
Methodology
Pulse labeling and immunoprecipitation were used to track Gag and ABCE1 interactions in primate cells.
Limitations
The study does not quantify how much Gag is present as Gag–ABCE1 complexes.
Digital Object Identifier (DOI)
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