Regulation of vascular endothelial growth factor expression in human colon cancer by interleukin-1β
1999
How interleukin-1β affects blood vessel growth in colon cancer
publication
Author Information
Author(s): Akagi Y, Liu W, Xie K, Zebrowski B, Shaheen R M, Ellis L M
Primary Institution: The University of Texas MD Anderson Cancer Center
Hypothesis
Does interleukin-1β induce vascular endothelial growth factor expression in human colon cancer cells?
Conclusion
Interleukin-1β increases the expression of vascular endothelial growth factor in human colon cancer cells by enhancing the transcription of the VEGF gene.
Supporting Evidence
- IL-1β treatment led to a peak induction of VEGF mRNA at 24 hours.
- VEGF expression was confirmed at the protein level after IL-1β treatment.
- The activity of the VEGF promoter was increased by IL-1β in transfected HT29 cells.
Takeaway
This study found that a substance called interleukin-1β helps cancer cells in the colon make more of a protein that helps grow blood vessels.
Methodology
HT29 and SW620 human colon cancer cells were treated with interleukin-1β, and VEGF expression was measured at the mRNA and protein levels.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website