SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines
2009
SET-NUP214 Fusion in Leukemia Cell Lines
Sample size: 141
publication
Evidence: moderate
Author Information
Author(s): Hilmar Quentmeier, Björn Schneider, Sonja Röhrs, Julia Romani, Margarete Zaborski, Roderick AF MacLeod, Hans G Drexler
Primary Institution: DSMZ-German Collection of Microorganisms and Cell Cultures
Hypothesis
Does the SET-NUP214 fusion gene contribute to the pathogenesis of T-cell acute lymphoblastic leukemia and acute myeloid leukemia?
Conclusion
Cell lines LOUCY and MEGAL express the SET-NUP214 fusion gene, making them promising models for further studies.
Supporting Evidence
- The SET-NUP214 fusion gene was found in only two of the 141 cell lines tested.
- Both LOUCY and MEGAL cell lines expressed the fusion protein.
- Genomic sequencing confirmed the presence of the fusion gene in both cell lines.
Takeaway
Scientists found a special gene fusion in some leukemia cell lines that might help them understand how leukemia works.
Methodology
Screening of 141 leukemia/lymphoma cell lines for SET-NUP214 expression using RT-PCR and genomic sequencing.
Digital Object Identifier (DOI)
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