Nuclear oxidative damage and colorectal cancer survival
Author Information
Author(s): Sheridan J, Wang L-M, Tosetto M, Sheahan K, Hyland J, Fennelly D, O'Donoghue D, Mulcahy H, O'Sullivan J
Primary Institution: Centre for Colorectal Disease, St Vincent's University Hospital
Hypothesis
The study examines the relationship between levels of 8-oxo-dG, a marker of oxidative DNA damage, and survival in colorectal cancer patients.
Conclusion
High levels of 8-oxo-dG are associated with poor survival outcomes in colorectal cancer patients.
Supporting Evidence
- Epithelial cytoplasmic staining of 8-oxo-dG was significantly greater in tumor tissue compared to normal tissue (P<0.001).
- High levels of nuclear 8-oxo-dG staining in normal mucosa were associated with poor survival (P=0.0003).
- Survival was unrelated to cytoplasmic staining in either tumor or normal mucosa.
Takeaway
This study found that a specific type of DNA damage, called 8-oxo-dG, can help predict how well people with colorectal cancer will do.
Methodology
The study assessed 8-oxo-dG levels in colorectal cancer tissues using immunohistochemistry and compared them with normal tissues.
Potential Biases
Potential biases may arise from the selection of patients and the retrospective nature of the study.
Limitations
The study may not account for all confounding factors affecting survival, and the cutoff points for 8-oxo-dG levels were arbitrarily defined.
Participant Demographics
Median age of colorectal cancer patients was 67 years, with 73 men and 70 women.
Statistical Information
P-Value
p<0.0001
Confidence Interval
95% CI 1.3–3.6
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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