Understanding the Effects of a Chemical on Yeast Cells
Author Information
Author(s): Danièle Kemmer, Lianne M. McHardy, Shawn Hoon, Delphine Rebérioux, Guri Giaever, Corey Nislow, Calvin D. Roskelley, Michel Roberge
Primary Institution: University of British Columbia
Hypothesis
Can combining different chemical genomics approaches provide a more complete understanding of drug action in yeast?
Conclusion
Combining various screening methods revealed that the chemical dihydromotuporamine C affects multiple cellular processes, including sphingolipid biosynthesis and mitochondrial function.
Supporting Evidence
- The study identified 51 chemicals that inhibited yeast growth.
- DhMotC was shown to require functional mitochondria to kill yeast cells.
- Chemical-genetic synthetic lethality screens revealed genes involved in vacuolar pH and vesicle-mediated transport.
Takeaway
Scientists used yeast to see how a chemical affects cells, finding that it can stop cells from growing and mess with their insides.
Methodology
The study used various chemical genomics approaches, including halo assays and liquid growth assays, to assess the effects of dihydromotuporamine C on yeast.
Limitations
The study primarily focused on yeast, which may not fully represent human cellular responses.
Digital Object Identifier (DOI)
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