Simultaneous determination of 50 antibiotic residues in plasma by HPLC–MS/MS
2024

Detecting 50 Antibiotic Residues in Plasma

Sample size: 80 publication Evidence: high

Author Information

Author(s): Hu Jun, Ba Yina, Pan Zhifeng, Li Xiaogang

Primary Institution: Shanghai Electric Power Hospital

Hypothesis

Can we develop a reliable method to detect multiple antibiotic residues in human plasma simultaneously?

Conclusion

The developed method can accurately detect 50 types of antibiotics in human plasma, demonstrating its feasibility and reliability.

Supporting Evidence

  • The method can detect antibiotics with a recovery rate between 67.25% and 129.03%.
  • Limit of detection values ranged from 0.1 ng/ml to 5 ng/ml for various antibiotics.
  • The method was validated on 80 patient samples, showing good application value.

Takeaway

Scientists created a new way to find 50 different antibiotics in blood quickly and accurately, which helps keep people safe from harmful drug residues.

Methodology

The study used HPLC-MS/MS to analyze plasma samples for antibiotic residues, optimizing sample preparation and chromatographic conditions.

Limitations

The method's accuracy may vary depending on the pH of specific antibiotics, which could influence recovery values.

Participant Demographics

Participants were healthy individuals enrolled from Shanghai Electric Power Hospital between March 2022 and June 2023.

Digital Object Identifier (DOI)

10.1016/j.heliyon.2024.e40629

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