Induction of Macrophage Chemotaxis by Aortic Extracts from Patients with Marfan Syndrome Is Related to Elastin Binding Protein Chemotaxis in Marfan Syndrome
2011

Macrophage Chemotaxis Induced by Aortic Extracts in Marfan Syndrome

Sample size: 25 publication Evidence: moderate

Author Information

Author(s): Guo Gao, Gehle Petra, Doelken Sandra, Martin-Ventura José Luis, von Kodolitsch Yskert, Hetzer Roland, Robinson Peter N.

Primary Institution: Charité-Universitätsmedizin Berlin

Hypothesis

Do aortic extracts from patients with Marfan syndrome induce macrophage chemotaxis?

Conclusion

Aortic extracts from patients with Marfan syndrome can induce macrophage chemotaxis, likely mediated by elastin binding proteins.

Supporting Evidence

  • Aortic extracts from Marfan patients showed significantly higher chemotactic activity compared to control samples.
  • The chemotactic response was inhibited by pretreatment with lactose or specific peptides.
  • There was a significant correlation between chemotactic index and BA4 reactivity.

Takeaway

Doctors studied samples from people with a condition called Marfan syndrome and found that these samples can attract certain immune cells, which might help explain some health problems related to the condition.

Methodology

Aortic samples were collected from patients and analyzed for their ability to induce macrophage chemotaxis using a modified Boyden chamber.

Limitations

Control aortic specimens were obtained from deceased heart donors, which may introduce confounding effects related to age or gender.

Participant Demographics

6 patients with Marfan syndrome (average age 38.5 years, 5 male) and 8 patients with isolated thoracic aortic aneurysm (average age 58.6 years, all male).

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0020138

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