Serotonin Enhances Gut Pacemaker Activity
Author Information
Author(s): Liu Hong-Nian, Ohya Susumu, Nishizawa Yuji, Sawamura Kenta, Iino Satoshi, Syed Mohsin Md, Goto Kazunori, Imaizumi Yuji, Nakayama Shinsuke
Primary Institution: Nagoya University Graduate School of Medicine
Hypothesis
Does serotonin (5-HT) enhance the pacemaker activity of interstitial cells of Cajal (ICC) in the gut?
Conclusion
The study found that serotonin significantly enhances the pacemaker activity of ICC via 5-HT3 receptors.
Supporting Evidence
- 5-HT significantly enhanced spontaneous Ca2+ oscillations in ICC.
- LY-278584, a 5-HT3 receptor antagonist, suppressed spontaneous Ca2+ activity in ICC.
- 2-methylserotonin, a 5-HT3 receptor agonist, restored Ca2+ activity in ICC.
- RT-PCR confirmed the expression of 5-HT3 receptors in ICC.
Takeaway
Serotonin helps the gut cells that control movement to work better, which could help with tummy troubles.
Methodology
The study used Ca2+ imaging and microelectrode array systems to measure ICC activity in isolated murine ileum muscle preparations.
Participant Demographics
BALB/c and W/Wv mice aged 3–6 weeks.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website