Flagellin Internalization by Intestinal Epithelium
Author Information
Author(s): Eaves-Pyles Tonyia, Bu Heng-Fu, Tan Xiao-di, Cong Yingzi, Patel Jignesh, Davey Robert A., Strasser Jane E.
Primary Institution: University of Texas Medical Branch
Hypothesis
Flagellin may also be internalized by intestinal epithelial cells following binding to apically expressed TLR5.
Conclusion
Luminal-applied flagellin is internalized by normal intestinal epithelial cells via TLR5 and co-localizes to endosomal and lysosomal compartments.
Supporting Evidence
- Flagellin was internalized by intestinal epithelial cell monolayers.
- Apical exposure to flagellin stimulated transepithelial migration of neutrophils and dendritic cells.
- Silencing or blocking TLR5 in Caco-2BBe cells resulted in a lack of flagellin internalization.
Takeaway
This study shows that a protein from bacteria called flagellin can enter intestinal cells and help the body respond to infections without harming the cells.
Methodology
The study used polarized intestinal epithelial cell lines and examined the internalization of flagellin and its effects on immune responses.
Limitations
The study primarily focused on in vitro models, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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