Suppression of manganese superoxide dismutase augments sensitivity to radiation, hyperthermia and doxorubicin in colon cancer cell lines by inducing apoptosis
2000
Targeting Mn-SOD to Enhance Cancer Treatment
publication
Author Information
Author(s): Kuninaka S, Ichinose Y, Koja K, Toh Y
Primary Institution: Clinical Research Institute, Department of Chest Surgery, Gastroenterologic Surgery, National Kyushu Cancer Center
Hypothesis
Is Mn-SOD a potential target to augment the sensitivity of cancer cells to various anti-cancer treatments?
Conclusion
Inhibiting Mn-SOD makes colon cancer cells with wild-type p53 more sensitive to treatments like radiation and doxorubicin.
Supporting Evidence
- Inhibition of Mn-SOD activity increases sensitivity to radiation, hyperthermia, and doxorubicin in colon cancer cells with wild-type p53.
- HCT116 antisense clones showed greater DNA fragmentation and apoptosis compared to controls.
- DLD1 antisense clones did not show enhanced sensitivity to the treatments.
Takeaway
Scientists found that blocking a certain enzyme in colon cancer cells helps them respond better to treatments that kill cancer.
Methodology
Stable Mn-SOD antisense RNA expressing cell clones were established from two human colon cancer cell lines, HCT116 and DLD1.
Digital Object Identifier (DOI)
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