Differential Requirements of Two recA Mutants for Constitutive SOS Expression in Escherichia coli K-12 recA Constitutive Mutants
2008
How Two Mutants of recA Affect SOS Response in E. coli
Sample size: 3000
publication
Evidence: moderate
Author Information
Author(s): Long Jarukit Edward, Renzette Nicholas, Centore Richard C., Sandler Steven J.
Primary Institution: University of Massachusetts at Amherst
Hypothesis
Different recA mutants express the SOS Response through varying mechanisms.
Conclusion
The study concludes that not all recAC alleles cause SOS expression by the same mechanism.
Supporting Evidence
- recA730 cells have a normal distribution and an average RFI of 40±7 units.
- recA4142 cells had an average RFI of about 3.6±0.7.
- Increasing the level of transcription of recA4142 may increase the proportion of cells with high level of SOSC expression.
Takeaway
Some bacteria have special proteins that help them fix their DNA when it gets damaged, and different versions of these proteins work in different ways.
Methodology
Genetic analysis of two recAC mutants was performed using fluorescence of single cells carrying an SOS reporter system.
Limitations
The specific model for the DNA substrates that recA730 binds to is not established.
Digital Object Identifier (DOI)
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