Detection of HbA2' Cases in Pakistan
Author Information
Author(s): Nusrat Maliha, Moiz Bushra, Nasir Amna, Rasool Hashmi Mashhooda
Primary Institution: The Aga Khan University Hospital
Hypothesis
Small S-window peaks on chromatography are secondary to the presence of HbA2' in the blood samples.
Conclusion
The study concluded that HbA2' was not detected in the Pakistani population, and small S-window peaks should not be reported as HbA2' without genetic confirmation.
Supporting Evidence
- 192 potential cases of HbA2' were detected on HPLC.
- 64 new cases were suspected of having co-existing β-thalassaemia trait.
- Molecular analysis showed no evidence of the HbA2' mutation in any sample.
Takeaway
The study looked for a specific type of hemoglobin in blood samples from Pakistan but found no evidence of it, suggesting that some test results might be misleading.
Methodology
The study used high performance liquid chromatography (HPLC) to analyze blood samples and confirmed findings with molecular techniques.
Potential Biases
Potential laboratory errors could have influenced the results.
Limitations
The study could not identify the true nature of small S-window peaks and did not evaluate exon 3 of the delta globin gene.
Participant Demographics
The study included blood samples from various regions of Pakistan.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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