Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells
2011

The Role of HOXD9 in Gliomas and Cancer Stem Cells

Sample size: 29 publication Evidence: moderate

Author Information

Author(s): Tabuse Masanao, Ohta Shigeki, Ohashi Yohei, Fukaya Raita, Misawa Aya, Yoshida Kazunari, Kawase Takeshi, Saya Hideyuki, Thirant Cécile, Chneiweiss Hérve, Matsuzaki Yumi, Okano Hideyuki, Kawakami Yutaka, Toda Masahiro

Primary Institution: Keio University School of Medicine

Hypothesis

HOXD9 contributes to cell proliferation and survival in gliomas and glioma cancer stem-like cells.

Conclusion

HOXD9 may serve as a novel marker for glioma cancer stem cells and a potential therapeutic target.

Supporting Evidence

  • HOXD9 was highly expressed in glioma cell lines and human glioma tissues.
  • Silencing HOXD9 led to decreased cell proliferation and induced apoptosis in glioma cells.
  • HOXD9 expression was higher in glioma cancer stem cells compared to normal astrocytes.

Takeaway

HOXD9 is a gene that helps glioma cells grow and survive, and targeting it could help treat brain tumors.

Methodology

The study involved quantitative real-time PCR, immunohistochemistry, and siRNA treatment to analyze HOXD9 expression and function in glioma cells.

Limitations

The study did not establish a direct correlation between HOXD9 expression and tumor grade or proliferation index.

Participant Demographics

Patients with glioma who underwent surgery at Keio University and Paris Descartes University.

Statistical Information

P-Value

2.93E-10

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1476-4598-10-60

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