Isolation of Ovulation-Inducing Factor from Llama Seminal Plasma
Author Information
Author(s): Ratto Marcelo H, Delbaere Louis TJ, Leduc Yvonne A, Pierson Roger A, Adams Gregg P
Primary Institution: University of Saskatchewan
Hypothesis
The study aimed to isolate and purify the protein fraction(s) of llama seminal plasma responsible for the ovulation-inducing effect of the ejaculate.
Conclusion
Ovulation-inducing factor was isolated from llama seminal plasma as a 14 kDa protein molecule that elicits a preovulatory LH surge followed by ovulation and CL formation in llamas.
Supporting Evidence
- Ovulation and CL formation was detected in 10/11 llamas treated with whole seminal plasma.
- A surge in circulating concentrations of LH was detected within 2 hours only in llamas treated with whole seminal plasma or Fraction C2.
- Plasma progesterone concentration and CL diameter profiles were greatest in llamas treated with Fraction C2.
Takeaway
Scientists found a special protein in llama semen that helps trigger ovulation in female llamas.
Methodology
Semen was collected from male llamas, and seminal plasma was processed using hydroxylapatite and gel filtration chromatography to isolate protein fractions.
Participant Demographics
55 non-lactating female llamas, ≥4 years of age and weighing 90-150 kg.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website