DNA methylation in the promoter region of the p16 (CDKN2/MTS-1/INK4A) gene in human breast tumours
1999
DNA Methylation in Breast Tumours
Sample size: 13
publication
Evidence: moderate
Author Information
Author(s): Woodcock D M, Linsenmeyer M E, Doherty J P, Warren W D
Primary Institution: Peter MacCallum Cancer Institute
Hypothesis
The study investigates the role of DNA methylation in the p16 gene in human breast tumours.
Conclusion
DNA methylation of the p16 gene in some breast tumours may result from an active process that could be targeted for therapy.
Supporting Evidence
- The p16 region was unmethylated in four normal breast specimens and in four out of nine breast tumours.
- A uniform pattern of DNA methylation was observed in five breast tumour specimens.
- Four of the nine major sites of DNA methylation were in non-CG sequences.
Takeaway
Some breast cancer cells change their DNA in a way that might help doctors find new treatments.
Methodology
Bisulphite genomic sequencing was used to examine DNA methylation in breast cancer specimens and normal breast tissue.
Participant Demographics
The study included human breast cancer specimens and normal breast tissue from reductive mammaplasty.
Digital Object Identifier (DOI)
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