Identification of a subpopulation of MeCCNU resistant cells in previously untreated Lewis lung tumours
1984

Resistance of Lewis Lung Tumors to MeCCNU

Sample size: 15 publication Evidence: moderate

Author Information

Author(s): T.C. Stephens, K. Adams, J.H. Peacock

Primary Institution: Institute of Cancer Research

Hypothesis

What is the extent of resistance to MeCCNU in previously untreated Lewis lung tumors?

Conclusion

A small subpopulation of tumor cells in Lewis lung tumors is resistant to MeCCNU, with about 1 in 105 cells showing this resistance.

Supporting Evidence

  • MeCCNU treatment showed a biphasic survival curve in Lewis lung tumors.
  • At low doses, the majority of tumor cells were highly sensitive to MeCCNU.
  • At high doses, the presence of resistant cells was suggested by the growth delay response.

Takeaway

Some cancer cells in Lewis lung tumors can survive a medicine called MeCCNU, making it harder to treat the tumors.

Methodology

Mice with Lewis lung tumors were treated with varying doses of MeCCNU, and tumor cell survival was assessed using excision cell survival assays and lung colony assays.

Limitations

The study does not clarify the nature or origin of the resistance in tumor cells.

Participant Demographics

C57B1/Cbi mice, weighing 20-25g.

Statistical Information

P-Value

p<0.05

Confidence Interval

95% CL 1.68-1.93

Statistical Significance

p<0.05

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