The clathrin-binding motif and the J-domain of Drosophila Auxilin are essential for facilitating Notch ligand endocytosis
2008
The Role of Drosophila Auxilin in Notch Ligand Endocytosis
publication
Evidence: high
Author Information
Author(s): Kandachar Vasundhara, Bai Ting, Chang Henry C
Primary Institution: Purdue University
Hypothesis
Drosophila Auxilin is essential for facilitating Notch ligand endocytosis.
Conclusion
Drosophila Auxilin mutations inhibit Notch ligand internalization, disrupting Notch signaling.
Supporting Evidence
- Mutations in dAux disrupt Notch-dependent processes.
- Delta accumulates at the cell surface in dAux mutant cells.
- dAux is required for the internalization of Notch ligands.
- Clathrin distribution is disrupted in dAux mutant clones.
- Over-expression of the clathrin-binding motif and J-domain can rescue dAux phenotypes.
Takeaway
Drosophila Auxilin helps cells take in signals that tell them how to grow and develop, and when it doesn't work, the cells can't get those signals properly.
Methodology
The study involved genetic analysis of Drosophila auxilin mutations and their effects on Notch signaling processes.
Digital Object Identifier (DOI)
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