Resistance to Neonicotinoid Insecticides in Aphids
Author Information
Author(s): Chris Bass, Alin M Puinean, Melanie Andrews, Penny Cutler, Miriam Daniels, Jan Elias, Verity Laura Paul, Andrew J Crossthwaite, Ian Denholm, Linda M Field, Stephen P Foster, Rob Lind, Martin S Williamson, Russell Slater
Primary Institution: Centre for Sustainable Pest and Disease Management, Rothamsted Research
Hypothesis
Is a mutation in the nicotinic acetylcholine receptor β subunit associated with resistance to neonicotinoid insecticides in Myzus persicae?
Conclusion
The mutation in the nAChR β1 subunit reduces the sensitivity of the receptor to neonicotinoids, representing the first example of field-evolved target-site resistance.
Supporting Evidence
- The resistant clone exhibited a significant reduction in binding affinity to imidacloprid.
- A single point mutation in the nAChR β1 subunit was identified in the resistant clone.
- The mutation causes an amino acid change that reduces sensitivity to neonicotinoids.
Takeaway
Some aphids have changed so they don't get sick from certain bug sprays anymore. This change is because of a tiny change in their body that makes the spray not work as well.
Methodology
The study involved bioassays, metabolism analysis, gene expression studies, and binding assays to investigate resistance mechanisms.
Limitations
The study primarily focused on one resistant clone and may not represent all populations of Myzus persicae.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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