Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers
Author Information
Author(s): Nathan A. Baird, Paul D. Etter, Tressa S. Atwood, Mark C. Currey, Anthony L. Shiver, Zachary A. Lewis, Eric U. Selker, William A. Cresko, Eric A. Johnson
Primary Institution: Institute of Molecular Biology, University of Oregon
Hypothesis
There remains a need for a simple, inexpensive platform that allows high-density SNP discovery and genotyping in large populations.
Conclusion
The RAD sequencing approach allows for rapid SNP discovery and genetic mapping in various organisms.
Supporting Evidence
- The RAD sequencing method identified more than 13,000 SNPs.
- The study successfully mapped traits in two model organisms.
- The approach allows for high-throughput genotyping of large populations.
Takeaway
Scientists found a quick way to discover genetic markers and map traits in fish and fungi using a new DNA sequencing method.
Methodology
The study used RAD sequencing to identify SNPs and map traits in two model organisms, threespine stickleback and Neurospora crassa.
Limitations
The study may not be applicable to organisms without a reference genome.
Participant Demographics
The study involved threespine stickleback fish from Alaskan populations and Neurospora crassa strains.
Statistical Information
P-Value
0.0007
Statistical Significance
p<0.0007
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website