How Varicellovirus Proteins Affect Antigen Processing
Author Information
Author(s): Koppers-Lalic Danijela, Verweij Marieke C., Lipińska Andrea D., Wang Ying, Quinten Edwin, Reits Eric A., Koch Joachim, Loch Sandra, Rezende Marisa Marcondes, Daus Franz, Bieńkowska-Szewczyk Krystyna, Osterrieder Nikolaus, Mettenleiter Thomas C., Heemskerk Mirjam H. M., Tampé Robert, Neefjes Jacques J., Chowdhury Shafiqul I., Ressing Maaike E., Rijsewijk Frans A. M., Wiertz Emmanuel J. H. J.
Primary Institution: Leiden University Medical Center
Hypothesis
The UL49.5 proteins of varicelloviruses inhibit the function of the transporter associated with antigen processing (TAP).
Conclusion
The UL49.5 proteins from various varicelloviruses inhibit TAP function through different mechanisms, affecting MHC class I antigen presentation.
Supporting Evidence
- The UL49.5 proteins of BHV-1, PRV, EHV-1, and EHV-4 were shown to inhibit TAP activity.
- BHV-1 UL49.5 targets TAP for proteasomal degradation, while EHV-1 and EHV-4 UL49.5 interfere with ATP binding to TAP.
- VZV UL49.5 interacts with TAP but does not inhibit peptide transport.
Takeaway
Some viruses have special proteins that stop our body's defenses from recognizing and fighting them. This study shows how these proteins work in different viruses.
Methodology
The study involved expressing UL49.5 proteins in various cell lines and assessing their impact on TAP function and MHC class I expression using flow cytometry and peptide transport assays.
Limitations
The study primarily focuses on specific varicelloviruses and may not generalize to all herpesviruses.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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