An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes
2009
Swt1: An RNA Endonuclease Linked to mRNP Quality Control
publication
Evidence: high
Author Information
Author(s): Michal Skružný, Claudia Schneider, Attila Rácz, Julan Weng, David Tollervey, Ed Hurt
Primary Institution: Biochemie-Zentrum der Universität Heidelberg (BZH), Heidelberg, Germany
Hypothesis
The conserved PIN domain protein Swt1 is an RNA endonuclease that participates in quality control of nuclear mRNPs and associates with the nuclear pore complex.
Conclusion
Swt1 is essential for degrading defective pre-mRNPs to prevent their export and translation.
Supporting Evidence
- Swt1 showed endoribonuclease activity in vitro that was inhibited by a point mutation.
- Inhibition of Swt1 increased levels of unspliced aberrant pre-mRNA.
- Swt1 accumulates at nuclear pore complexes in mutants with NPC clustering.
- Loss of Swt1 leads to increased cytoplasmic leakage of splicing-defective pre-mRNAs.
Takeaway
Swt1 is like a cleanup crew for messy RNA in the cell, making sure only the good stuff gets sent out.
Methodology
The study involved genetic interaction tests, in vitro nuclease activity assays, and RNA localization analyses.
Digital Object Identifier (DOI)
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