Fast and Sensitive E. coli Detection Using a Paper-Based Biosensor
Author Information
Author(s): Soysaldı Furkan, Dincyurek Ekici Derya, Soylu Mehmet Çağrı, Mutlugun Evren
Primary Institution: Biological and Medical Diagnostic Sensors Laboratory (BioMeD Sensors Lab), Department of Biomedical Engineering, Erciyes University, Kayseri, Türkiye
Hypothesis
Can a hybrid biosensor combining electrochemical and optical detection methods improve the sensitivity and speed of E. coli detection?
Conclusion
The developed hybrid biosensor can detect E. coli at a low concentration of 2.7 × 102 CFU/mL within 30 minutes.
Supporting Evidence
- The biosensor achieved a limit of detection of 2.7 × 102 CFU/mL for E. coli.
- Quantum dot fluorescence showed over 60% lifetime quenching as E. coli concentration increased.
- The sensor can perform both electrochemical and optical measurements simultaneously.
- The relative standard deviation (RSD) of the sensor was found to be 3.5781%, indicating good repeatability.
Takeaway
This study created a special sensor that can quickly find E. coli bacteria using both electrical and light methods, making it very fast and accurate.
Methodology
The biosensor was created using paper, pencil, and magnetic quantum dots, and it detects E. coli through electrochemical impedance spectroscopy and optical fluorescence.
Limitations
The study did not conduct additional external cross-check experiments.
Digital Object Identifier (DOI)
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