Understanding the ARTS Protein and Its Interaction with XIAP
Author Information
Author(s): Reingewertz Tali H., Shalev Deborah E., Sukenik Shahar, Blatt Ofrah, Rotem-Bamberger Shahar, Lebendiker Mario, Larisch Sarit, Friedler Assaf
Primary Institution: Institute of Chemistry, Hebrew University of Jerusalem
Hypothesis
The study investigates the molecular details of the interaction between the intrinsically disordered C-terminus of the ARTS protein and the Bir3 domain of XIAP.
Conclusion
The ARTS protein's C-terminal domain is intrinsically disordered and binds to the Bir3 domain of XIAP, which is crucial for its pro-apoptotic function.
Supporting Evidence
- ARTS is a pro-apoptotic protein that functions as a tumor suppressor.
- ARTS binds and inhibits XIAP by interacting with its Bir3 domain.
- The interaction between ARTS and Bir3 is crucial for promoting apoptosis.
Takeaway
The ARTS protein helps cells die when they need to, and it does this by sticking to another protein called XIAP. This study shows how they connect.
Methodology
Biophysical and computational methods, including NMR and fluorescence spectroscopy, were used to analyze the interaction between ARTS CTD and Bir3.
Digital Object Identifier (DOI)
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