Isoflurane's Effect on Protein Structure
Author Information
Author(s): Michelle R. Baker, Sean K. Benton, Christopher S. Theisen, Chad A. McClintick, Eugene E. Fibuch, Norbert W. Seidler
Primary Institution: University of Missouri-Kansas City School of Medicine
Hypothesis
Does isoflurane binding alter the conformation of human serum albumin and its susceptibility to chemical modification?
Conclusion
Isoflurane binding leads to persistent changes in protein conformation and increased susceptibility to chemical modifications.
Supporting Evidence
- Isoflurane binding leads to a persistent change in protein conformation.
- Increased susceptibility to chemical modification was observed after isoflurane treatment.
- The study suggests a novel mechanism for preconditioning related to isoflurane's effects.
Takeaway
Isoflurane, a type of anesthetic, changes how a protein called human serum albumin looks and behaves, making it more likely to react with other chemicals.
Methodology
The study involved preincubating human serum albumin with isoflurane, followed by dialysis and various assays to assess protein conformation and susceptibility to chemical modification.
Statistical Information
P-Value
p<0.0005
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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