Ubiquitin and SUMO Conjugation in Alzheimer's Disease Model
Author Information
Author(s): Laura E. McMillan, Jon T. Brown, Jeremy M. Henley, Helena Cimarosti
Primary Institution: University of Bristol
Hypothesis
Altered levels of SUMO-conjugated substrates might be evident in Tg2576 mice.
Conclusion
The study found increased levels of ubiquitinated proteins in the hippocampus of Tg2576 mice, while SUMO conjugation levels remained unchanged.
Supporting Evidence
- Global levels of ubiquitinated proteins increased in the hippocampus of Tg2576 mice.
- No global changes in either SUMO-1 or SUMO-2/3 conjugation in any brain regions analysed.
- Total levels of AMPA and kainate receptors were also unaffected in Tg2576 mice.
Takeaway
This study looked at how certain proteins change in a mouse model of Alzheimer's disease, finding that some proteins were tagged for degradation more than others.
Methodology
The study used Western blotting to analyze protein levels in brain regions of Tg2576 transgenic mice and wild-type controls.
Limitations
The study did not identify the specific proteins that were increasingly conjugated to ubiquitin.
Participant Demographics
9-month male Tg2576 mice and age-matched wild-type controls.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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