Camptothecin sensitizes androgen-independent prostate cancer cells to anti-Fas-induced apoptosis
1999
Camptothecin Helps Prostate Cancer Cells Die
publication
Author Information
Author(s): Costa-Pereira A P, Cotter T G
Primary Institution: Tumour Biology Laboratory, Department of Biochemistry, University College, Ireland
Hypothesis
Can camptothecin make androgen-independent prostate cancer cells more sensitive to anti-Fas-induced apoptosis?
Conclusion
Camptothecin can sensitize certain prostate cancer cells to undergo cell death when treated with anti-Fas.
Supporting Evidence
- DU145 prostate cancer cells were resistant to anti-Fas-induced cell death.
- Camptothecin pretreatment overcame resistance in DU145 cells.
- Activated caspases were required for camptothecin-mediated cytotoxicity.
- Poly(ADP-Ribose) polymerase was cleaved in cells treated with camptothecin plus anti-Fas.
- ZVAD completely blocked camptothecin-mediated Fas-induced apoptosis.
- Sensitization did not involve up-regulation of Fas or FasL.
- Reactive oxygen intermediates were not altered by camptothecin treatment.
Takeaway
This study found that a drug called camptothecin can help some prostate cancer cells die when combined with another treatment.
Methodology
The study involved treating prostate cancer cells with camptothecin and anti-Fas to observe effects on cell death.
Digital Object Identifier (DOI)
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