Comet assay analysis of single–stranded DNA breaks in circulating leukocytes of glaucoma patients
2008

DNA Damage in Glaucoma Patients

Sample size: 56 publication Evidence: moderate

Author Information

Author(s): Mozaffarieh M, Schoetzau A, Sauter M, Grieshaber M, Orgül S, Golubnitschaja O, Flammer J

Primary Institution: University Eye Clinic, Basel, Switzerland

Hypothesis

Is there an association between primary vascular dysregulation and DNA breaks in glaucoma patients?

Conclusion

POAG patients with PVD have a significantly higher rate of DNA breaks than both POAG patients without PVD and healthy controls.

Supporting Evidence

  • Leukocytes of POAG patients had significantly more comet tails than controls.
  • DNA breaks were particularly high in POAG patients with primary vascular dysregulation.
  • No significant difference in DNA breaks was found between healthy controls with and without PVD.

Takeaway

People with a type of eye disease called glaucoma have more broken DNA in their blood cells, especially if they also have blood flow problems.

Methodology

The study compared DNA breaks in blood cells of glaucoma patients and healthy controls using the comet assay.

Participant Demographics

The study included POAG patients and healthy controls, matched by age and sex.

Statistical Information

P-Value

p=0.002

Statistical Significance

p<0.001

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