Notch3 Is Dispensable for Thymocyte β-Selection and Notch1-Induced T Cell Leukemogenesis
2011

Notch3's Role in T Cell Development and Leukemogenesis

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Author Information

Author(s): Suliman Sara, Tan Joanne, Xu Keli, Kousis Philaretos C., Kowalski Paul E., Chang Greg, Egan Sean E., Guidos Cynthia

Primary Institution: Hospital for Sick Children Research Institute

Hypothesis

Is Notch3 necessary for thymocyte β-selection and Notch1-induced T cell leukemogenesis?

Conclusion

Notch3 is not required for thymocyte β-selection or for T cell leukemogenesis induced by Notch1.

Supporting Evidence

  • N3-deficient DN3 thymocytes were not defective in generating DP thymocytes.
  • N3 deficiency did not exacerbate the competitive defect of N1+/− DN3 progenitors.
  • N3 deficiency did not alter the incidence or latency of T-LL induced by ICN1.

Takeaway

The study found that Notch3 is not needed for T cell development or cancer caused by Notch1, even though it is present in T cells.

Methodology

Mice lacking Notch3 were generated and analyzed for T cell development and leukemogenesis.

Limitations

The study primarily focused on the role of Notch3 without exploring potential compensatory mechanisms by other Notch receptors.

Statistical Information

P-Value

p=0.4

Statistical Significance

p=0.4

Digital Object Identifier (DOI)

10.1371/journal.pone.0024937

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