Widespread Over-Production of Pyocyanin in Cystic Fibrosis Strain
Author Information
Author(s): Fothergill Joanne L, Panagea Stavroula, Hart Charles A, Walshaw Martin J, Pitt Tyrone L, Winstanley Craig
Primary Institution: University of Liverpool
Hypothesis
What is the prevalence of the unusual pyocyanin over-production phenotype among isolates of the cystic fibrosis epidemic strain?
Conclusion
The widespread occurrence of the unusual pyocyanin over-production phenotype suggests it may play an important role in the success of the epidemic strain.
Supporting Evidence
- 51% of the LES isolates produced increased pyocyanin compared to strain PA01.
- The unusual phenotype can persist for up to seven years during chronic infection.
- There was a correlation between the OP phenotype and resistance to ceftazidime, aztreonam, and meropenem.
Takeaway
Some bacteria that cause lung infections in cystic fibrosis patients make too much of a harmful substance called pyocyanin, which can make the infection worse.
Methodology
The study screened 34 non-LES and 55 LES isolates for pyocyanin production using a simple broth test and assessed antimicrobial susceptibilities.
Limitations
The study may not account for all variations in pyocyanin production due to different growth conditions.
Participant Demographics
The study involved adult and child CF patients from Liverpool, with 21 male and 13 female non-LES isolates and 10 male and 6 female LES isolates.
Statistical Information
P-Value
p = 0.004
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website