Comparing Polyadenylation Signals in Humans and Flies
Author Information
Author(s): Retelska Dorota, Iseli Christian, Bucher Philipp, Jongeneel C Victor, Naef Felix
Primary Institution: Swiss Institute of Bioinformatics
Hypothesis
How do polyadenylation signals differ between humans and Drosophila melanogaster?
Conclusion
The study found that while the polyadenylation signal is highly conserved between humans and flies, the downstream sequence element shows more divergence.
Supporting Evidence
- The polyadenylation signal is highly conserved in both species.
- De novo motif searches refined the description of the downstream sequence element.
- Model assessment showed that including the downstream sequence element improves accuracy of predictions.
Takeaway
This study looked at how genes in humans and flies use similar signals to cut and add parts to their RNA. They found that some parts are very similar, but others are different.
Methodology
The study used a Hidden Markov Model to analyze over 1.5 million human and 30,000 Drosophila ESTs to compare polyadenylation signals.
Limitations
The study may not account for all possible polyadenylation signals due to the limitations of the datasets used.
Digital Object Identifier (DOI)
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