TDP-43 knockdown impairs neurite outgrowth dependent on its target histone deacetylase 6
2011

TDP-43 Knockdown Affects Neurite Growth

publication Evidence: moderate

Author Information

Author(s): Fiesel Fabienne C, Schurr Christine, Weber Stephanie S, Kahle Philipp J

Primary Institution: Hertie Institute for Clinical Brain Research, University of Tuebingen

Hypothesis

Does TDP-43 knockdown impair neurite outgrowth through its target HDAC6?

Conclusion

TDP-43 deficiency leads to impairment of neurite growth in an HDAC6-dependent manner.

Supporting Evidence

  • TDP-43 knockdown significantly impairs neurite outgrowth.
  • Re-transfection with TDP-43 and HDAC6 rescues neurite outgrowth.
  • Silencing HDAC6 alone reduces neurite outgrowth.

Takeaway

When a protein called TDP-43 is reduced, it makes nerve cells grow less, which can be bad for brain health.

Methodology

The study used human neuroblastoma SH-SY5Y cells to assess neurite outgrowth after silencing TDP-43 and HDAC6.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1750-1326-6-64

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