Reconstitution of Blood Cells After Bone Marrow Transplant in Monkeys
Author Information
Author(s): Derdouch Sonia, Gay Wilfried, Nègre Didier, Prost Stéphane, Le Dantec Mikael, Delache Benoît, Auregan Gwenaelle, Andrieu Thibault, Leplat Jean-Jacques, Cosset François-Loïc, Le Grand Roger
Primary Institution: CEA, service d'Immuno-Virologie, Institut des Maladies Emergentes et Thérapies Innovantes, Direction des Sciences du Vivant, Fontenay aux Roses, France
Hypothesis
Can autologous CD34+ bone marrow cells improve blood cell reconstitution after gamma irradiation in cynomolgus macaques?
Conclusion
Transplanting CD34+ bone marrow cells helps restore various blood cell types in monkeys after radiation treatment.
Supporting Evidence
- Blood cells producing eGFP were detected as early as three days after transplantation.
- eGFP-producing granulocyte and mononuclear cells persisted for more than one year in the periphery.
- Transduction rates did not differ significantly between colony-forming cells and immature cells.
Takeaway
When monkeys received special bone marrow cells after radiation, their blood cells grew back better.
Methodology
Cynomolgus macaques were irradiated and then received autologous CD34+ bone marrow cells that were genetically modified.
Potential Biases
Potential bias due to small sample size and the specific animal model used.
Limitations
The study involved a small number of animals and one animal died during the study.
Participant Demographics
Male cynomolgus macaques, weighing between 3 and 6 kg.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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