Effects of Calcium and NPS R-467 on Equine Stem Cells
Author Information
Author(s): Martino Nicola Antonio, Lange-Consiglio Anna, Cremonesi Fausto, Valentini Luisa, Caira Michele, Guaricci Antonio Ciro, Ambruosi Barbara, Sciorsci Raffaele Luigi, Lacalandra Giovanni Michele, Reshkin Stephan Joel, Dell'Aquila Maria Elena
Primary Institution: Department of Animal Production, Faculty of Biotechnological Sciences, University of Bari, Valenzano, Bari, Italy
Hypothesis
The study investigates how high external calcium concentration and the calcimimetic NPS R-467 affect the growth and proliferation of equine umbilical cord matrix mesenchymal stem cells.
Conclusion
Calcium and the calcimimetic NPS R-467 reduce CaSR mRNA expression and stimulate cell growth/proliferation in equine umbilical cord matrix stem cells.
Supporting Evidence
- Increased external calcium concentration significantly increased cell growth in the small cell line.
- NPS R-467 significantly increased cell growth in both cell lines.
- Preincubation with the CaSR antagonist NPS 2390 inhibited the agonistic effect of NPS R-467.
Takeaway
This study shows that adding calcium and a specific drug can help equine stem cells grow faster, which is important for getting enough cells for research or treatment.
Methodology
The study involved culturing two equine size-sieved umbilical cord matrix mesenchymal stem cell lines in different calcium concentrations and with the addition of NPS R-467, followed by analysis of cell growth and CaSR expression.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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