Gene Expression Differences in Prenatal Muscle Development of Pigs
Author Information
Author(s): Tang Zhonglin, Li Yong, Wan Ping, Li Xiaoping, Zhao Shuhong, Liu Bang, Fan Bin, Zhu Mengjin, Yu Mei, Li Kui
Primary Institution: Chinese Academy of Agricultural Sciences
Hypothesis
What are the molecular mechanisms underlying differences in muscle growth between Tongcheng and Landrace pigs during prenatal development?
Conclusion
The study found significant differences in gene expression related to muscle growth between Tongcheng and Landrace pigs, with Landrace pigs showing faster muscle growth.
Supporting Evidence
- The study sequenced 317,115 LongSAGE tags and identified 1,400 and 1,201 differentially expressed transcripts in Tongcheng and Landrace pigs, respectively.
- Growth factors and genes involved in protein synthesis were up-regulated in Landrace pigs.
- Twelve differentially expressed genes were validated by quantitative PCR.
Takeaway
Scientists looked at how pig muscles grow before they are born and found that different types of pigs grow muscles in different ways.
Methodology
The study used long serial analysis of gene expression (LongSAGE) to analyze muscle tissue from pigs at three prenatal stages.
Limitations
Many LongSAGE tags did not match known sequences, indicating gaps in the porcine genome database.
Participant Demographics
The study involved Tongcheng and Landrace pig breeds, with equal representation from both breeds.
Statistical Information
P-Value
8.52E-17
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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