Localization of hRad9 in Breast Cancer
Author Information
Author(s): Chan Vivian, Khoo US, Wong MS, Lau Ken, Suen Dacita, Li George, Kwong Ava, Chan TK
Primary Institution: University Department of Medicine, Queen Mary Hospital, Hong Kong, China
Hypothesis
The study aims to define the role of hRad9 in breast carcinogenesis through immunohistochemical studies.
Conclusion
Finding of hyperphosphorylated forms of hRad9 in the nucleus of cancer cells is in keeping with its function in ameliorating DNA instability, whereby it inadvertently assists tumor growth.
Supporting Evidence
- Increased hRad9 protein was observed in breast cancer cells nucleus compared to non-tumor epithelium.
- The nuclear score found in tumor samples with increased hRad9 mRNA was significantly higher compared to those with normal hRad9 mRNA level.
- The study used 37 sets of breast tumor and adjacent normal breast tissues for analysis.
Takeaway
This study found that a protein called hRad9 is more active in the nuclei of breast cancer cells, which might help the cancer grow by fixing DNA problems.
Methodology
Immunohistochemical studies and Western blotting were performed on paired tumor and normal breast tissues.
Statistical Information
P-Value
< 0.001
Statistical Significance
p < 0.001
Digital Object Identifier (DOI)
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