SVIP Regulates p97/VCP Localization and Autophagy
Author Information
Author(s): Wang Yang, Ballar Petek, Zhong Yongwang, Zhang Xuebao, Liu Chao, Zhang Ying-Jiu, Monteiro Mervyn J., Li Jun, Fang Shengyun
Primary Institution: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, People's Republic of China
Hypothesis
Does SVIP play a regulatory role in p97/VCP localization and autophagy?
Conclusion
SVIP enhances autophagy by regulating p97/VCP localization and increasing LC3 lipidation and p62 expression.
Supporting Evidence
- SVIP overexpression leads to localization of p97/VCP at the plasma membrane and lysosomal membranes.
- Knockdown of SVIP reduces LC3 lipidation and p62 protein levels.
- SVIP enhances starvation-induced autophagy and degradation of p62.
Takeaway
SVIP helps move a protein called p97/VCP to where it needs to be in the cell, which helps the cell clean up and recycle its parts.
Methodology
The study involved overexpression and knockdown of SVIP in HeLa cells, followed by immunoblotting and immunofluorescence to analyze protein localization and autophagy markers.
Digital Object Identifier (DOI)
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